Metabolic control analysis of Escherichia coli mutants for the fed-batch production of L-tryptophan

Kristin Schoppel, doctoral thesis Technical University of Munich, 2022

Metabolic control analyses of recombinant Escherichia coli transporter strains enabled the identification of controlling reaction steps of L-tryptophan biosynthesis based on glycerol. The resulting targeted genetically modified mutants showed an altered metabolic control distribution and improved production characteristics. Extended metabolite analyses provided for the first time insights into metabolic reactions that led to the premature end of L-tryptophan production during the fed-batch process.

Publications

  • Schoppel K, Trachtmann N, Korzin EJ, Tzanavari A, Sprenger GA, Weuster-Botz D (2022): Rational strain design based on metabolic control analysis improved L-tryptophan production with E. coli until methylglyoxal formation stops biosynthesis. Microb Cell Fact 21: 201.
  • Schoppel K, Trachtmann N, Mittermeier F, Sprenger G, Weuster-Botz D (2021): Metabolic control analysis of L-tryptophan producing Escherichia coli applying targeted perturbation with shikimate. Bioproc Biosys Eng 44: 2591-2613.
  • Tröndle J, Schoppel K, Bleidt A, Trachtmann N, Sprenger GA, Weuster-Botz D (2020): Metabolic control analysis of Escherichia coli producing L-tryptophan from glycerol based on data from a parallelized short-term perturbation experiment. J Biotechnol 307: 15-28.