Metabolic control analysis of Escherichia coli mutants for the fed-batch production of L-tryptophan

Kristin Schoppel, doctoral thesis Technical University of Munich, 2022

Metabolic control analyses of recombinant Escherichia coli transporter strains enabled the identification of controlling reaction steps of L-tryptophan biosynthesis based on glycerol. The resulting targeted genetically modified mutants showed an altered metabolic control distribution and improved production characteristics. Extended metabolite analyses provided for the first time insights into metabolic reactions that led to the premature end of L-tryptophan production during the fed-batch process.


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