Membrane functionalization of nano-scale enzyme membrane reactors for asymmetric syntheses

Tom Schwarzer, doctoral thesis Technische Universität München, 2017

Polymeric nano-compartments with encapsulated enzymes can be used as nano-scale enzyme membrane reactors. Mass transfer limitations can be alleviated by integrating natural channel proteins into the membrane. In this thesis, the very limited repertoire of channel proteins functionally integrated into polymer membranes was extended by six new channel proteins suitable for the transport of substrates for the asymmetric synthesis of chiral alcohols.


  • Schubeis T, Schwarzer TS, Le Marchand T, Stanek J, Tekwani Movellan K, Castiglione K, Pintacuda G, Andreas LB (2020): Resonance assignment of the outer membrane protein AlkL in lipid bilayers by proton-detected solid-state NMR. Biomol NMR Assign 14, 295–300.
  • Schubeis T, Le Marchand T, Daday C, Kopec W, Tekwani Movellan K, Stanek J, Schwarzer TS, Castiglione K, de Groot BL, Pintacuda G, Andreas LB (2020): A β-barrel for oil transport through lipid membranes: Dynamic NMR structures of AlkL. Proc Nat Acad Sci 117: 21014-21021.
  • Najbauer EE Movellan KT, Schubeis T, Schwarzer T, Castiglione K, Giller K, Pintacuda G, Becker S, Andreas LB (2019): Probing membrane protein insertion into lipid bilayers by solid-state NMR. ChemPhysChem 20: 302 –310.
  • Schwarzer TS, Klermund L, Wang G, Castiglione K (2018): Membrane functionalization of polymersomes: allevating mass transport limitations by integrating multiple selective membrane transporters for the diffusion of chemically diverse molecules. Nanotechnol 29: 44LT01.
  • Jain MG, Lalli D, Stanek J, Gowda C, Prakash S, Schwarzer TS, Schubeis T, Castiglione K, Andreas LB, Madhu PK, Pintacuda G, Agarwal V (2017): Selective 1H–1H Distance Restraints in Fully Protonated Proteins by Very Fast Magic-Angle Spinning Solid-State NMR. J Phys Chem Lett 8: 2399-2405.
  • Schwarzer TS, Hermann M, Krishnan S, Simmel FC, Castiglione K (2017): Preparative refolding of small monomeric outer membrane proteins. Prot Expres Purif 132 (2017): 171-181.