Reaction engineering analysis of D-galacturonic acid reduction to L-galactonate with recombinant Saccharomyces cerevisiae

Jacqueline Wagner, doctoral thesis Technical University of Munich, 2021

The biocatalytic activity of recombinant Saccharomyces cerevisiae with different reductases, cofactor regeneration enzymes, and transport proteins was characterized comparatively in controlled stirred tank reactors. Cofactor regeneration with sorbitol and overexpressed sorbitol dehydrogenase allowed for the first time a complete conversion of D-galacturonic acid to L-galactonate with whole cell biocatalysts in a batch process with defined medium and clarified sugar beet press pulp hydrolysate.

Publications

• Wagner J, Schäfer D, von den Eichen N, Haimerl C, Harth S, Oreb M, Benz JP, Weuster-Botz D (2021): D-galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate. Appl Microbiol Biotechnol 105: 5795–5807.
• Harth S, Wagner J, Sens T, Choe J, Benz P, Weuster-Botz D, Oreb M (2020): Engineering cofactor supply and NADH-dependent D-galacturonic acid reductases for redox-balanced production of L-galactonate in Saccharomyces cerevisiae. Scientific Reports 10: 19021.