Reaction engineering analysis of single-stranded DNA production with bacteriophage M13

Benjamin Kick, Doctoral Thesis Technical University of Munich, 2017


Scaffolded DNA origami enables the fabrication of three dimensional nanostructures using single-stranded DNA (ssDNA). Varying processes were investigated in stirred-tank bioreactors to enable the scalable production of ssDNA from the bacteriophage M13 after infection of appropriate Escherichia coli strains. Highest concentrations of up to 590 mg L-1 ssDNA isolated from the produced bacteriophages were achieved in high-cell density fermentation with Escherichia coli in fed-batch bioprocesses.



  • Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz H (2017): Biotechnological mass production of DNA origami. Nature 552: 84-87.    
  • Kick B, Behler KL, Severin TS, Weuster-Botz D (2017): Chemostat studies of bacteriophage M13 infected Escherichia coli JM 109 for continuous ssDNA production. J Biotechnol  258: 92-100.
  • Kick B, Hensler S, Praetorius F, Dietz H, Weuster-Botz D (2017): Specific growth rate and multiplicity of infection affect high-cell density fermentation with bacteriophage M13 for ssDNA production. Biotechnol Bioeng 114: 777–784
  • Kick B, Praetorius F, Dietz H, Weuster-Botz D (2015): Efficient production of single-stranded phage DNA as scaffolds for DNA origami. Nano Letters 15: 4672-4676.