Scalable process for the production of nano-compartments made of ABA-triblock copolymers

Sarah Poschenrieder, doctoral thesis Technische Universität München, 2017

Polymeric nano-compartments can be used as artificial reaction chambers for biotransformations showing no side-reactions. A one-step process was developed in miniaturized stirred-tank reactors to produce spherical polymer vesicles with narrow size distribution. This process was successfully scaled-up to the liter-scale by the use of the dimensionless Froude number. Thus polymeric nanovesicles with low polydispersity could be produced within 1.5 hours in a batch process.

Publications

Poschenrieder ST, Hanzlik M, Castiglione K (2018): Polymersome formation mechanism and formation rate in stirred-tank reactors. J Appl Polym Sci, DOI 10.1002/app.46077.
Poschenrieder ST, Schiebel SK, Castiglione K (2018): Stability of polymersomes with focus on their use as nanoreactors. Eng Life Sci 18: 101-113.
Poschenrieder ST, Klermund L, Langer B, Castiglione K (2017): Determination of Permeability Coefficients of Polymersomal Membranes for Hydrophilic Molecules. Langmuir, 33: 6011-6020.
Klermund L, Poschenrieder TS, Castiglione K (2017): Biocatalysis in Polymersomes: Improving Multienzyme Cascades with Incompatible Reaction Steps by Compartmentalization. ACS Catal 7: 3900-3904.
Poschenrieder ST, Schiebel SK, Castiglione K (2017): Polymersomes for biotechnological applications: Large-scale production of nano-scale vesicles. Eng Life Sci 17: 58–70.
Klermund L, Poschenrieder ST, Castiglione K (2016): Simple surface functionalization of polymersomes using non-antibacterial peptide anchors. J Nanobiotechnol 14: 48.
Poschenrieder ST, Wagner SG, Castiglione K (2016): Efficient production of uniform nanometer-sized polymer vesicles in stirred-tank reactors. J Appl Polym Sci 133: 43274.