Biphasic whole-cell production of geranyl glucoside by recombinant Escherichia coli

Xenia Priebe, doctoral thesis Technical University of Munich, 2019

Escherichia coli cells expressing a plant glycosyltransferase were used as whole-cell biocatalysts for the glycosylation of geraniol. A rational selected biphasic system with the non-aqueous phase serving as an in situ substrate reservoir was applied. By providing an oxygen-abundant reaction environment and by deleting the enzyme responsible for undesired byproduct formation, geranyl glucoside was produced in high and so far unrivaled concentrations and yields in stirred-tank bioreactors.

Publications

• Priebe X, Hoang MD, Rüdiger J, Turgel M, Tröndle J, Schwab W, Weuster-Botz D (2020): Byproduct free geraniol glycosylation by whole-cell biotransformation with recombinant Escherichia coli. Biotechnol Lett 43: 247–259.
• Priebe X, Daugulis AJ (2018): Thermodynamic affinity-based considerations for the rational selection of biphasic systems for microbial flavor and fragrance production. J Chem Technol Biotechnol 93: 656-666.
• Priebe X, Daschner M, Schwab W, Weuster-Botz D (2018): Rational selection of biphasic reaction systems for geranyl glucoside production by Escherichia coli whole-cell biocatalysts. Enzyme Microb Technol 112: 79-87
• Schmideder A, Priebe X, Rubenbauer M, Hoffmann T, Huang F-C, Schwab W, Weuster-Botz D (2016): Non-water miscible ionic liquid improves biocatalytic production of geranyl glucoside with Escherichia coli overexpressing a glucosyltransferase. Bioproc Biosys Eng 39: 1409-1414.