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Technical University of Munich

Production of D-xylitol from sugar beet press pulp with Aspergillus niger

Melanie Knesebeck, doctoral thesis Technical University of Munich, 2025

Integrated enzyme production with A. niger and the following enzymatic hydrolysis of sugar beet press pulp were achieved with high yields and sugar concentrations in a solid-state bioreactor. The L-arabinose in the clarified hydrolysate could only be used after activated carbon treatment by another genetically modified A. niger strain to produce D-xylitol in a stirred tank bioreactor with yields of up to 45%, with the other sugars of the hydrolysate being metabolized.

Publications
  • Knesebeck M, Rüllke M, Schönrock V, Benz JP, Weuster-Botz D (2024): Xylitol production from sugar beet press pulp hydrolysat with engineered Aspergillus niger. Microorganisms 12: 2489.
  • Regmi P, Knesebeck M, Boles E, Weuster-Botz D, Oreb M (2024): A comparative analysis of NADPH supply strategies in Saccharomyces cerevisiae: Production of D-xylitol from D-xylose as a case study. Metab Eng Comm 19: e00245.
  • Knesebeck M, Schäfer D, Schmitz K, Rüllke M, Benz JP, Weuster-Botz D (2023): Enzymatic one-pot hydrolysis of extracted sugar beet press pulp after solid-state fermentation with an engineered Aspergillus niger strain. Fermentation 9: 582.
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