Integrated enzyme production with A. niger and the following enzymatic hydrolysis of sugar beet press pulp were achieved with high yields and sugar concentrations in a solid-state bioreactor. The L-arabinose in the clarified hydrolysate could only be used after activated carbon treatment by another genetically modified A. niger strain to produce D-xylitol in a stirred tank bioreactor with yields of up to 45%, with the other sugars of the hydrolysate being metabolized.
Publications
- Knesebeck M, Rüllke M, Schönrock V, Benz JP, Weuster-Botz D (2024): Xylitol production from sugar beet press pulp hydrolysat with engineered Aspergillus niger. Microorganisms 12: 2489.
- Regmi P, Knesebeck M, Boles E, Weuster-Botz D, Oreb M (2024): A comparative analysis of NADPH supply strategies in Saccharomyces cerevisiae: Production of D-xylitol from D-xylose as a case study. Metab Eng Comm 19: e00245.
- Knesebeck M, Schäfer D, Schmitz K, Rüllke M, Benz JP, Weuster-Botz D (2023): Enzymatic one-pot hydrolysis of extracted sugar beet press pulp after solid-state fermentation with an engineered Aspergillus niger strain. Fermentation 9: 582.